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Restricted access of proteins to mannan polysaccharides in intact plant cell walls

Lookup NU author(s): Professor Liam Donaldson, Dr Artur Rogowski, Emeritus Professor Harry Gilbert, Professor William WillatsORCiD


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P>How the diverse polysaccharides present in plant cell walls are assembled and interlinked into functional composites is not known in detail. Here, using two novel monoclonal antibodies and a carbohydrate-binding module directed against the mannan group of hemicellulose cell wall polysaccharides, we show that molecular recognition of mannan polysaccharides present in intact cell walls is severely restricted. In secondary cell walls, mannan esterification can prevent probe recognition of epitopes/ligands, and detection of mannans in primary cell walls can be effectively blocked by the presence of pectic homogalacturonan. Masking by pectic homogalacturonan is shown to be a widespread phenomenon in parenchyma systems, and masked mannan was found to be a feature of cell wall regions at pit fields. Direct fluorescence imaging using a mannan-specific carbohydrate-binding module and sequential enzyme treatments with an endo-beta-mannanase confirmed the presence of cryptic epitopes and that the masking of primary cell wall mannan by pectin is a potential mechanism for controlling cell wall micro-environments.

Publication metadata

Author(s): Marcus SE, Blake AW, Benians TAS, Lee KJD, Poyser C, Donaldson L, Leroux O, Rogowski A, Petersen HL, Boraston A, Gilbert HJ, Willats WGT, Knox JP

Publication type: Article

Publication status: Published

Journal: Plant Journal

Year: 2010

Volume: 64

Issue: 2

Pages: 191-203

Print publication date: 23/07/2010

ISSN (print): 0960-7412

ISSN (electronic): 1365-313X

Publisher: Wiley-Blackwell Publishing Ltd.


DOI: 10.1111/j.1365-313X.2010.04319.x


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Funder referenceFunder name
BB/E014364/1UK Biotechnology and Biological Sciences Research Council
BB/G024898/1UK Biotechnology and Biological Sciences Research Council