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Lookup NU author(s): Dr Mark Velangi, Elizabeth Matheson, Dr Andrew Hall, Professor Julie Irving
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Defects in the mismatch repair system are associated with a microsatellite unstable phenotype. In this chapter, we describe the preparation of purified plasma cells using CD138 magnetic microbeads as a source of tumor DNA. We also describe a robust, sensitive method for comparing microsatellite repeat units of tumor to constitutive DNA using polymerase chain reaction and laser scanning of fluorescently labeled amplicons in an automated sequencer in order to assess microsatellite instability in myeloma.
Author(s): Velangi M, Matheson E, Hall AG, Irving JAE
Editor(s): Brown RD; Ho PJ
Publication type: Book Chapter
Publication status: Published
Book Title: Multiple Myeloma
Year: 2005
Volume: 113
Pages: 269-77
Series Title: Methods in Molecular Medicine
Publisher: Humana Press, Inc.
Place Published: Totowa, N.J
URL: http://www.springer.com/medicine/internal/book/978-1-58829-392-3
Library holdings: Search Newcastle University Library for this item
ISBN: 9781588293923
