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Detecting mismatch repair defects in myeloma

Lookup NU author(s): Dr Mark Velangi, Elizabeth Matheson, Emeritus Professor Andy Hall, Professor Julie Irving

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Abstract

Defects in the mismatch repair system are associated with a microsatellite unstable phenotype. In this chapter, we describe the preparation of purified plasma cells using CD138 magnetic microbeads as a source of tumor DNA. We also describe a robust, sensitive method for comparing microsatellite repeat units of tumor to constitutive DNA using polymerase chain reaction and laser scanning of fluorescently labeled amplicons in an automated sequencer in order to assess microsatellite instability in myeloma.


Publication metadata

Author(s): Velangi M, Matheson E, Hall AG, Irving JAE

Editor(s): Brown RD; Ho PJ

Publication type: Book Chapter

Publication status: Published

Book Title: Multiple Myeloma

Year: 2005

Volume: 113

Pages: 269-77

Series Title: Methods in Molecular Medicine

Publisher: Humana Press, Inc.

Place Published: Totowa, N.J

URL: http://www.springer.com/medicine/internal/book/978-1-58829-392-3

Library holdings: Search Newcastle University Library for this item

ISBN: 9781588293923


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