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Crystallization and preliminary X-ray analysis of the bacillaene synthase trans-acting acyltransferase PksC

Lookup NU author(s): Dr Fiona Cuskin, Dr Alexandra Solovyova, Professor Rick Lewis, Dr Paul Race

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Abstract

The antibiotic bacillaene is biosynthesized in Bacillus subtilis by a hybrid type 1 modular polyketide synthase/nonribosomal peptide synthetase of the trans-acyltransferase (trans-AT) class. Within this system, the essential acyl-group loading activity is provided by the action of three free-standing trans-acting acyltransferases. Here, the recombinant expression, purification and crystallization of the bacillaene synthase trans-acting acyltransferase PksC are reported. A diffraction data set has been collected from a single PksC crystal to 1.44 A resolution and the crystal was found to belong to the orthorhombic space group P2(1)2(1)2(1).


Publication metadata

Author(s): Cuskin F, Solovyova AS, Lewis RJ, Race PR

Publication type: Article

Publication status: Published

Journal: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online

Year: 2011

Volume: 67

Issue: 4

Pages: 464-466

Print publication date: 01/04/2011

ISSN (print):

ISSN (electronic): 1744-3091

Publisher: Wiley-Blackwell Publishing, Inc.

URL: http://dx.doi.org/10.1107/S1744309111003484

DOI: 10.1107/S1744309111003484


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