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Sequence requirements of ATF2 and CREB binding to the human T-cell leukemia virus type 1 LTR R region

Lookup NU author(s): Professor Olaf Heidenreich


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We have identified crucial transcription factor contact sites within the distal portion of the HTLV-1 LTR R region. Single base substitutions within this region greatly reduce formation of a complex which we previously described as a 70-kDa nuclear protein interacting with a CREB-like protein. Comparison of published sequences of HTLV-1 isolates obtained from scattered geographic locations revealed that clustered mutations in an 8-base segment near the U5 junction do, in fact, occur naturally. A single base substitution corresponding to a common naturally occurring mutation was introduced into the R region of an HTLV-1-LTR Cat construct. This resulted in derepression of the promoter in a cell line expressing high levels of the R region binding complex when compared to the wild-type LTR promoter. Affinity purification and electrophoretic mobility super-shift analysis identified a dominant 70-kDa DNA binding protein as ATF-2. Phosphorylated ATF-2 apparently interacts with CREB to form this downstream complex.

Publication metadata

Author(s): Xu X, Kang SH, Heidenreich O, Brown DA, Nerenberg MI

Publication type: Article

Publication status: Published

Journal: Virology

Year: 1996

Volume: 218

Issue: 2

Pages: 362-371

ISSN (print): 0042-6822

ISSN (electronic): 1089-862X


DOI: 10.1006/viro.1996.0205


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