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Development and validation of a standardized protocol to monitor human dietary exposure by metabolite fingerprinting of urine samples

Lookup NU author(s): Dr Gaelle Fave, Graham Harold, Dr Long Xie, Professor John Mathers


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Conventional tools for measuring dietary exposure have well recognized limitations. Measurement of food-derived metabolites in biofluids provides an alternative approach and our aim was to develop an experimental protocol which ensures that extraneous variability does not obscure metabolic signals from ingested foods. Healthy adults consumed a standardized meal in the evening before each test day and collected pooled overnight urine. On each test day of three different studies, urine was collected in the fasted state and at different time points after consumption of a standardized breakfast. Metabolite fingerprinting of samples using Flow Infusion Electrospray-Ionization Mass Spectrometry followed by multivariate data analysis showed strong discrimination between overnight, fasting and postprandial samples, in each study separately and when data from the three studies were pooled. Such differences were robust and highly reproducible within individuals on separate occasions. Urine volume was an efficient data normalization factor for metabolite fingerprinting data. Postprandial urines had a stable chemical composition over a period of 2-4 h after eating a standardized breakfast, suggesting that there is a flexible time window for urine collection. Fasting urine samples provided a stable baseline for universal comparisons with postprandial samples. A dietary exposure biomarker discovery protocol was validated by demonstrating that top-ranked signals discriminating between fasting and 2-4 h postprandial urine samples could be linked to metabolites abundant in some components of the standardized breakfast. We conclude that the protocol developed will have value in the search for biomarker leads of dietary exposure.

Publication metadata

Author(s): Fave G, Beckmann M, Lloyd AJ, Zhou SB, Harold G, Lin WC, Tailliart K, Xie L, Draper J, Mathers JC

Publication type: Article

Publication status: Published

Journal: Metabolomics

Year: 2011

Volume: 7

Issue: 4

Pages: 469-484

Print publication date: 22/02/2011

ISSN (print): 1573-3882

ISSN (electronic): 1573-3890

Publisher: Springer


DOI: 10.1007/s11306-011-0289-0


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Funder referenceFunder name
N05073UK Food Standards Agency