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Lookup NU author(s): Professor Steve Homans
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Here we present a suite of pulse sequences for the measurement of 15N T1, T1ρ and NOE data that combine traditional TROSY-based pulse sequences with band-selective Hadamard frequency encoding. The additive nature of the Hadamard matrix produces much reduced resonance overlap without the need for an increase in the dimensionality of the experiment or a significant decrease in the signal to noise ratio. We validate the accuracy of these sequences in application to ubiquitin and demonstrate their utility for relaxation measurements in Escherichia coli Class II fructose 1,6-bisphosphate aldolase (FBP-aldolase), a 358 residue 78 kDa dimeric enzyme.
Author(s): Burnley BT, Kalverda AP, Paisey SJ, Berry A, Homans SW
Publication type: Article
Publication status: Published
Journal: Journal of Biomolecular NMR
ISSN (print): 0925-2738
ISSN (electronic): 1573-5001
Publisher: Springer Netherlands
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