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Lookup NU author(s): Professor Steve Homans
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NMR dipole-dipole couplings between protein backbone nuclei (1H, 13C, 15N, 1HN,13C) offer enormous scope for the rapid determination of protein global folds. Here, we show that measurement of one-bond splittings in the protein backbone is facilitated by use of protein that is selectively isotopically enriched only in the backbone atoms. In particular, 1H-13C couplings can be measured simply and with high sensitivity by use of conventional heteronuclear single quantum correlation (HSQC) techniques.
Author(s): Giesen AW, Bae LC, Barrett CL, Chyba JA, Chaykovsky MM, Cheng MC, Murray JH, Oliver EJ, Sullivan SM, Brown JM, Dahlquist FW, Homans SW
Publication type: Article
Publication status: Published
Journal: Journal of Biomolecular NMR
ISSN (print): 0925-2738
ISSN (electronic): 1573-5001
Publisher: Springer Netherlands
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