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Lookup NU author(s): Professor Steve Homans
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By use of NMR residual dipolar coupling measurements in a dilute liquid-crystalline solvent, the solution structure has been determined of the complex between the oligosaccharide moiety of globotriaosylceramide (Gb3-OS) and the B-subunit homopentamer of verotoxin 1 (VTB). The dipolar coupling data indicate that Gb3-OS binds in a single binding site per monomer, which is identical to one of three sites inferred from the X-ray structure of the same complex. We find no evidence within experimental error for occupancy at either of the two additional binding sites observed per monomer in the crystal structure.
Author(s): Thompson GS, Shimizu H, Homans SW, Donohue-Rolfe A
Publication type: Article
Publication status: Published
Journal: Biochemistry
Year: 2000
Volume: 39
Issue: 43
Pages: 13153-13156
ISSN (print): 0006-2960
ISSN (electronic): 1520-4995
Publisher: American Chemical Society
URL: http://dx.doi.org/10.1021/bi001394+
DOI: 10.1021/bi001394+
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