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Stable-Isotope-Assisted NMR Studies on 13C-Enriched Sialyl Lewisx in Solution and Bound to E-Selectin

Lookup NU author(s): Professor Steve Homans


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We report the determination of the solution structure and dynamics of 13C-enriched Neu5Acα2−3Galβ1−4(Fucα1−3)GlcNAc (sialyl Lewisx), together with its bound-state conformation in association with E-selectin. The availability of 13C-enriched material enabled both the measurement of trans-glycosidic 13C−13C coupling constants as conformational probes in the tetrasaccharide and the application of three-dimensional nuclear Overhauser effect 13C−1H heteronuclear single-quantum correlation experiments for the measurement of intramolecular transferred nuclear Overhauser effects (TRNOEs) characterizing the bound-state conformation of the ligand. The additional dispersion afforded by the third (13C) dimension permitted straightforward identification and quantitation of these TRNOEs, and several TRNOEs were identified that had not previously been reported. The bound-state conformation of the ligand, determined by use of a heteronuclear full-relaxation matrix analysis of the TRNOE data, differs significantly from that reported in previous studies.

Publication metadata

Author(s): Harris R, Kiddle GR, Field RA, Milton MJ, Ernst B, Magnani JL, Homans SW

Publication type: Article

Publication status: Published

Journal: Journal of the American Chemical Society

Year: 1999

Volume: 121

Issue: 11

Pages: 2546-2551

Print publication date: 05/03/1999

ISSN (print): 0002-7863

ISSN (electronic): 1520-5126

Publisher: American Chemical Society


DOI: 10.1021/ja983423y


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