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Structure of the Phosphosaccharide-Inositol Core of the Leishmania-Donovani Lipophosphoglycan

Lookup NU author(s): Professor Steve Homans


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The phosphosaccharide-inositol core of the lipophosphoglycan of Leishmania donovani was generated by treatment of the glycoconjugate with mild acid and digestion with phosphatidylinositol-specific phospholipase C. The core was purified and examined by oneand two-dimensional 'H-'H NMR and by methylation analysis. From the results, the carbohydrate core was elucidated as a phosphosaccharide attached to the inositol residue of the lyso-alkylphosphatidylinositol anchor of lipophosphoglycaans follows: PO4+6Galp(ul-r6)Gal,(u1+3)Gal~(u1+3)Manp(al+3)Man~(al+4)Glc-Np(a1+6)rnyo-inositol. The presence of an internal galactofuranose residue is highly unusual and the Manp(ul~4)GlcNp(al-+6)myo-inositosleq uence is homologous to the respective portion of the glycosylphosphatidylinositol anchors reported for both the Trypanosoma brucei variant surface glycoprotein and the rat brain Thy1- glycoprotein.

Publication metadata

Author(s): Turco SJ, Orlandi PA, Homans SW, Ferguson MAJ, Dwek RA, Rademacher TW

Publication type: Article

Publication status: Published

Journal: Journal of Biological Chemistry

Year: 1989

Volume: 264

Issue: 12

Pages: 6711-6715

Print publication date: 25/04/1989

ISSN (print): 0021-9258

Publisher: American Society for Biochemistry and Molecular Biology, Inc.