Browse by author
Lookup NU author(s): Professor John Hesketh
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
3' Untranslated regions (3'UTRs) of messenger RNAs have important roles in post-transcriptional regulation of gene expression and this is partly achieved through binding of specific proteins to sequences or structures within these regions. Previously, replacement of a native luciferase 3'UTR with the human albumin 3'UTR has been found to lead to a 10-fold increase in luciferase reporter activity. In this work we investigated protein binding to the human albumin 3'UTR. Electrophoretic mobility shift and UV cross-linking assays indicate that a 50 kDa protein from Chinese Hamster Ovary (CHO) cells binds to the albumin 3'UTR, and affinity experiments followed by proteomics identified this protein as CUG binding protein 1 (CUG-BP1, also known as CELF1). Deletion analysis of the albumin 3'UTR showed that nucleotides 1-50 and nucleotides 101-150 are not required for binding but that removal of nucleotides 51-100 caused a loss in binding. The results suggest that CUG-BP1 binds to nucleotides 51-100 of the human albumin 3'UTR. In human cells CUG-BP1 binding may thus play a role in regulation of albumin expression and, additionally, it may have a function in post-transcriptional control in CHO cells. (C) 2012 Elsevier Inc. All rights reserved.
Author(s): Khaziapoul S, Pearson MJ, Pryme IF, Stern B, Hesketh JE
Publication type: Article
Publication status: Published
Journal: Biochemical and Biophysical Research Communications
Year: 2012
Volume: 426
Issue: 4
Pages: 539-543
Print publication date: 05/10/2012
ISSN (print): 0006-291X
ISSN (electronic): 1090-2104
Publisher: Elsevier Inc.
URL: http://dx.doi.org/10.1016/j.bbrc.2012.08.123
DOI: 10.1016/j.bbrc.2012.08.123
Altmetrics provided by Altmetric
