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Lookup NU author(s): Dr Max Temple,
Emeritus Professor Harry Gilbert
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The rumen anaerobic cellulolytic bacterium Eubacterium cellulosolvens produces a large range of cellulases and hemicellulases responsible for the efficient hydrolysis of plant cell wall polysaccharides. One of these enzymes, endoglucanase Cel5A, comprises a tandemly repeated carbohydrate-binding module (CBM65) fused to a glycoside hydrolase family 5 (Cel5A) catalytic domain, joined by flexible linker sequences. The second carbohydrate-binding module located at the C-terminus side of the endoglucanase (CBM65B) has been co-crystallized with either cellohexaose or xyloglucan heptasaccharide. The crystals belong to the hexagonal space group P6(5) and tetragonal space group P4(3)2(1)2, containing a single molecule in the asymmetric unit. The structures of CBM65B have been solved by molecular replacement.
Author(s): Venditto I, Basle A, Luis AS, Temple MJ, Ferreira LMA, Fontes CMGA, Gilbert HJ, Najmudin S
Publication type: Article
Publication status: Published
Journal: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
Print publication date: 01/02/2013
ISSN (print): 1744-3091
Publisher: Wiley-Blackwell Publishing, Inc.
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