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Lookup NU author(s): Dr Laura JardineORCiD, Ashley Ames-Draycott, Sarah Pagan, Dr Sharon Cookson, Dr Gavin Spickett, Professor Muzlifah Haniffa, Professor Matthew CollinORCiD, Dr Venetia BigleyORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Dendritic cells (DCs) and monocytes are critical regulators and effectors of innate and adaptive immune responses. Monocyte expansion has been described in many pathological states while monocyte and DC deficiency syndromes are relatively recent additions to the catalog of human primary immunodeficiency disorders. Clinically applicable screening tests to diagnose and monitor these conditions are lacking. Conventional strategies for identifying human DCs and monocytes have been based on the use of a lineage gate to exclude lymphocytes, thus preventing simultaneous detection of DCs, monocytes, and lymphocyte subsets. Here we demonstrate that CD4 is a reliable lineage marker for the human peripheral blood antigen-presenting cell compartment that can be used to identify DCs and monocytes in parallel with lymphocytes. Based on this principle, simple modification of a standard lymphocyte phenotyping assay permits simultaneous enumeration of four lymphocyte and five DC/monocyte populations from a single sample. This approach is applicable to clinical samples and facilitates the diagnosis of DC and monocyte disorders in a wide range of clinical settings, including genetic deficiency, neoplasia, and inflammation.
Author(s): Jardine L, Barge D, Ames-Draycott A, Pagan S, Cookson S, Spickett G, Haniffa M, Collin M, Bigley V
Publication type: Article
Publication status: Published
Journal: Frontiers in Immunology
Year: 2013
Volume: 4
Issue: 4
Print publication date: 27/12/2013
Online publication date: 27/12/2013
Acceptance date: 17/12/2013
Date deposited: 06/07/2015
ISSN (electronic): 1664-3224
Publisher: Frontiers Research Foundation
URL: http://dx.doi.org/10.3389/fimmu.2013.00495
DOI: 10.3389/fimmu.2013.00495
PubMed id: PMC3873601
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