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Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen-presenting cell compartment

Lookup NU author(s): Dr Laura Jardine, Ashley Ames-Draycott, Sarah Pagan, Dr Sharon Cookson, Dr Gavin Spickett, Professor Muzlifah Haniffa, Professor Matthew Collin, Dr Venetia Bigley

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

Dendritic cells (DCs) and monocytes are critical regulators and effectors of innate and adaptive immune responses. Monocyte expansion has been described in many pathological states while monocyte and DC deficiency syndromes are relatively recent additions to the catalog of human primary immunodeficiency disorders. Clinically applicable screening tests to diagnose and monitor these conditions are lacking. Conventional strategies for identifying human DCs and monocytes have been based on the use of a lineage gate to exclude lymphocytes, thus preventing simultaneous detection of DCs, monocytes, and lymphocyte subsets. Here we demonstrate that CD4 is a reliable lineage marker for the human peripheral blood antigen-presenting cell compartment that can be used to identify DCs and monocytes in parallel with lymphocytes. Based on this principle, simple modification of a standard lymphocyte phenotyping assay permits simultaneous enumeration of four lymphocyte and five DC/monocyte populations from a single sample. This approach is applicable to clinical samples and facilitates the diagnosis of DC and monocyte disorders in a wide range of clinical settings, including genetic deficiency, neoplasia, and inflammation.


Publication metadata

Author(s): Jardine L, Barge D, Ames-Draycott A, Pagan S, Cookson S, Spickett G, Haniffa M, Collin M, Bigley V

Publication type: Article

Publication status: Published

Journal: Frontiers in Immunology

Year: 2013

Volume: 4

Issue: 4

Print publication date: 27/12/2013

Online publication date: 27/12/2013

Acceptance date: 17/12/2013

ISSN (electronic): 1664-3224

Publisher: Frontiers Research Foundation

URL: http://dx.doi.org/10.3389/fimmu.2013.00495

DOI: 10.3389/fimmu.2013.00495

PubMed id: PMC3873601


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