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Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules

Lookup NU author(s): Emeritus Professor Harry Gilbert

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Abstract

Plant cell walls are complex configurations of polysaccharides that are recalcitrant to degradation. The enzymes deployed by microbes to degrade these materials comprise glycoside hydrolases, polysaccharide lyases, carbohydrate esterases and polysaccharide oxidases. Non-catalytic carbohydrate-binding modules (CBMs) are found as discretely folded units within the multi-modular structures of these enzymes where they play critical roles in the recognition of plant cell wall components and potentiating the activity of the enzymes. Here we propose a refinement to the Types A, B, and C classification of CBMs whereby the Type A CBMs remain those that bind the surfaces of crystalline polysaccharides but the Type B CBMs are redefined as those that bind internally on glycan chains (endo-type), CBMs that bind to the termini of glycan chains are defined as Type C modules (exo-type). In this context, we discuss recent advances, primarily driven by structural studies, which reveal the molecular modes of CBM-sugar interactions and how this specifically underpins and influences the biological function of CBMs in cell wall recognition and degradation.

Publication metadata

Author(s): Gilbert HJ, Knox JP, Boraston AB

Publication type: Article

Publication status: Published

Journal: Current Opinion in Structural Biology

Year: 2013

Volume: 23

Issue: 5

Pages: 669-677

Print publication date: 01/10/2013

Online publication date: 13/06/2013

ISSN (print): 0959-440X

ISSN (electronic): 1879-033X

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/j.sbi.2013.05.005

DOI: 10.1016/j.sbi.2013.05.005

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