Toggle Main Menu Toggle Search

Open Access padlockePrints

Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome

Lookup NU author(s): Thabata Alvarez, Emeritus Professor Harry Gilbert


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


In recent years, owing to the growing global demand for energy, dependence on fossil fuels, limited natural resources and environmental pollution, biofuels have attracted great interest as a source of renewable energy. However, the production of biofuels from plant biomass is still considered to be an expensive technology. In this context, the study of carbohydrate-binding modules (CBMs), which are involved in guiding the catalytic domains of glycoside hydrolases for polysaccharide degradation, is attracting growing attention. Aiming at the identification of new CBMs, a sugarcane soil metagenomic library was analyzed and an uncharacterized CBM (CBM_E1) was identified. In this study, CBM_E1 was expressed, purified and crystallized. X-ray diffraction data were collected to 1.95 angstrom resolution. The crystals, which were obtained by the sitting-drop vapourdiffusion method, belonged to space group I23, with unit-cell parameters a = b = c = 88.07 angstrom.

Publication metadata

Author(s): Campos BM, Alvarez TM, Liberato MV, Polikarpov I, Gilbert HJ, Zeri ACD, Squina FM

Publication type: Article

Publication status: Published

Journal: Acta Crystallographica Section F: Structural Biology Communications

Year: 2014

Volume: 70

Issue: 9

Pages: 1232-1235

Print publication date: 01/09/2014

Online publication date: 27/08/2014

Acceptance date: 02/07/2014

ISSN (print): 1744-3091

ISSN (electronic): 2053-230X

Publisher: Wiley-Blackwell


DOI: 10.1107/S2053230X14015520


Altmetrics provided by Altmetric