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Mitochondrial DNA as a Biosensor of UV Exposure in Human Skin.

Lookup NU author(s): Dr Amy Bowman, Professor Mark Birch-Machin

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Abstract

Mitochondrial DNA (mtDNA) has been demonstrated to be a reliable biomarker of UV-induced genetic damage in both animal and human skin. Properties of the mitochondrial genome which allow for its use as a biomarker of damage include its presence in multiple copies within a cell, its limited repair mechanisms, and its lack of protective histones. To measure UV-induced mtDNA damage (particularly in the form of strand breaks), real-time quantitative PCR (qPCR) is used, based on the observation that PCR amplification efficiency is decreased in the presence of high levels of damage. Here, we describe the measurement of UV-induced mtDNA damage, including the extraction of cellular DNA, qPCR to determine the relative amount of mtDNA, qPCR to determine UV-induced damage within a long strand of mtDNA, and the verification of the amplification process using gel electrophoresis.


Publication metadata

Author(s): Bowman A, Birch-Machin MA

Editor(s): Weissig, V; Edeas, M

Publication type: Book Chapter

Publication status: Published

Book Title: Mitochondrial Medicine

Year: 2015

Volume: 1265

Pages: 379-388

Print publication date: 14/03/2015

Online publication date: 06/01/2015

Acceptance date: 29/08/2014

Series Title: Methods in Molecular Biology

Publisher: Springer

Place Published: New York

URL: http://dx.doi.org/10.1007/978-1-4939-2288-8_27

DOI: 10.1007/978-1-4939-2288-8_27

PubMed id: 25634289

Library holdings: Search Newcastle University Library for this item

ISBN: 9781493922871


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