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The co-chaperone p23 promotes prostate cancer motility and metastasis

Lookup NU author(s): Dr Luke Gaughan, Professor Craig Robson, Professor Rakesh Heer

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

Prostate cancer is an androgen receptor (AR)-dependent malignancy at initiation and progression, therefore hormone therapy is the primary line of systemic treatment. Despite initial disease regression, tumours inevitably recur and progress to an advanced castration-resistant state a major feature of which is metastasis to the bone. Upregulation of AR cofactors and chaperones that overcome low hormone conditions to maintain basal AR activity has been postulated as a mechanism of therapy relapse.p23, an essential component of the apo-AR complex, acts also after ligand binding to increase AR transcriptional activity and target gene expression, partly by increasing chromatin-loaded holo-receptor-complexes. Immunohistochemical studies have demonstrated increased p23 expression in advanced prostate cancer. Here, we further characterise p23 roles in AR signalling and show that it modulates cytosolic AR levels in the absence of hormone, confirming a chaperoning function in the aporeceptor complex and suggesting p23 upregulates AR signalling at multiple stages. Moreover, p23 protein levels significantly increased upon treatment with not only androgen but also clinically relevant anti-androgens. This was in contrast to the HSP90 inhibitor 17-AAG, which did not modulate expression of the cochaperone important given the HSP90-independent roles we and others have previously described for p23.Further, we demonstrate p23 is implicated in prostate cancer cell motility and in acquisition of invasiveness capacity through the expression of specific genes known to participate in cancer progression. This may drive metastatic processes in vivo since analysis of prostate tumour biopsies revealed that high nuclear p23 significantly correlated with shorter survival times and with development of metastases in patients with lower grade tumours. We propose that increased p23 expression may allow cells to acquire a more aggressive phenotype, contributing to disease progression, and that p23 is a plausible secondary target in combination with HSP90 inhibition as a potential therapy for advanced prostate cancer. (C) 2014 Published by Elsevier B.V. on behalf of Federation of European Biochemical Societies.


Publication metadata

Author(s): Cano LQ, Lavery DN, Sin S, Spanjaard E, Brooke GN, Tilman JD, Abroaf A, Gaughan L, Robson CN, Heer R, Mauri F, de Rooij J, Driouch K, Bevan CL

Publication type: Article

Publication status: Published

Journal: Molecular Oncology

Year: 2015

Volume: 9

Issue: 1

Pages: 295-308

Print publication date: 01/01/2015

Online publication date: 06/09/2014

Acceptance date: 27/08/2014

Date deposited: 19/08/2015

ISSN (print): 1574-7891

ISSN (electronic): 1878-0261

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/j.molonc.2014.08.014

DOI: 10.1016/j.molonc.2014.08.014


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