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Lookup NU author(s): Dr Fiona Cuskin,
Emeritus Professor Harry Gilbert
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
The large bowel microbiota, a complex ecosystem resident within the gastrointestinal tract of all human beings and large mammals, functions as an essential, nonsomatic metabolic organ, hydrolysing complex dietary polysaccharides and modulating the host immune system to adequately tolerate ingested antigens. A significant member of this community, Bacteroides thetaiotaomicron, has evolved a complex system for sensing and processing a wide variety of natural glycoproducts in such a way as to provide maximum benefit to itself, the wider microbial community and the host. The immense ability of B. thetaiotaomicron as a ` glycan specialist' resides in its enormous array of carbohydrate-active enzymes, many of which are arranged into polysaccharide-utilization loci (PULs) that are able to degrade sugar polymers that are often inaccessible to other gut residents, notably alpha-mannan. The B. thetaiotaomicron genome encodes ten putative alpha-mannanases spread across various PULs; however, little is known about the activity of these enzymes or the wider implications of alpha-mannan metabolism for the health of both the microbiota and the host. In this study, SAD phasing of a selenomethionine derivative has been used to investigate the structure of one such B. thetaiotaomicron enzyme, BT2949, which belongs to the GH76 family of alpha-mannanases. BT2949 presents a classical (alpha/alpha)(6)-barrel structure comprising a large extended surface cleft common to other GH76 family members. Analysis of the structure in conjunction with sequence alignments reveals the likely location of the catalytic active site of this noncanonical GH76.
Author(s): Thompson AJ, Cuskin F, Spears RJ, Dabin J, Turkenburg JP, Gilbert HJ, Davies GJ
Publication type: Article
Publication status: Published
Journal: Acta Crystallographica Section D Biological Crystallography
Print publication date: 01/02/2015
Acceptance date: 01/12/2014
Date deposited: 26/08/2015
ISSN (print): 0907-4449
ISSN (electronic): 1399-0047
Publisher: Wiley-Blackwell Publishing, Inc,
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