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Lookup NU author(s): Dr Paul Milne, Dr Venetia BigleyORCiD, Dr Merry Gunawan, Professor Muzlifah Haniffa, Professor Matthew CollinORCiD
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Langerhans cells (LCs) are self-renewing in the steady state but repopulated by myeloid precursors after injury. Human monocytes give rise to langerin-positive cells in vitro, suggesting a potential precursor role. However, differentiation experiments with human lineage-negative cells and CD34(+) progenitors suggest that there is an alternative monocyte-independent pathway of LC differentiation. Recent data in mice also show long-term repopulation of the LC compartment with alternative myeloid precursors. Here we show that, although monocytes are able to express langerin, when cultured with soluble ligands granulocyte macrophage colony-stimulating factor (GM-CSF), transforming growth factor beta (TGF beta), and bone morphogenetic protein 7 (BMP7), CD1c(+) dendritic cells (DCs) become much more LC-like with high langerin, Birbeck granules, EpCAM, and E-cadherin expression under the same conditions. These data highlight a new potential precursor function of CD1c(+) DCs and demonstrate an alternative pathway of LC differentiation that may have relevance in vivo.
Author(s): Milne P, Bigley V, Gunawan M, Haniffa M, Collin M
Publication type: Article
Publication status: Published
Journal: Blood
Year: 2015
Volume: 125
Issue: 3
Pages: 470-473
Print publication date: 15/01/2015
Online publication date: 28/10/2014
Acceptance date: 14/10/2014
ISSN (print): 0006-4971
ISSN (electronic): 1528-0020
Publisher: American Society of Hematology
URL: http://dx.doi.org/10.1182/blood-2014-08-593582
DOI: 10.1182/blood-2014-08-593582
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