Toggle Main Menu Toggle Search

Open Access padlockePrints

CD1c+ blood dendritic cells have Langerhans cell potential

Lookup NU author(s): Dr Paul Milne, Dr Venetia BigleyORCiD, Dr Merry Gunawan, Professor Muzlifah Haniffa, Professor Matthew CollinORCiD


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Langerhans cells (LCs) are self-renewing in the steady state but repopulated by myeloid precursors after injury. Human monocytes give rise to langerin-positive cells in vitro, suggesting a potential precursor role. However, differentiation experiments with human lineage-negative cells and CD34(+) progenitors suggest that there is an alternative monocyte-independent pathway of LC differentiation. Recent data in mice also show long-term repopulation of the LC compartment with alternative myeloid precursors. Here we show that, although monocytes are able to express langerin, when cultured with soluble ligands granulocyte macrophage colony-stimulating factor (GM-CSF), transforming growth factor beta (TGF beta), and bone morphogenetic protein 7 (BMP7), CD1c(+) dendritic cells (DCs) become much more LC-like with high langerin, Birbeck granules, EpCAM, and E-cadherin expression under the same conditions. These data highlight a new potential precursor function of CD1c(+) DCs and demonstrate an alternative pathway of LC differentiation that may have relevance in vivo.

Publication metadata

Author(s): Milne P, Bigley V, Gunawan M, Haniffa M, Collin M

Publication type: Article

Publication status: Published

Journal: Blood

Year: 2015

Volume: 125

Issue: 3

Pages: 470-473

Print publication date: 15/01/2015

Online publication date: 28/10/2014

Acceptance date: 14/10/2014

ISSN (print): 0006-4971

ISSN (electronic): 1528-0020

Publisher: American Society of Hematology


DOI: 10.1182/blood-2014-08-593582


Altmetrics provided by Altmetric


Funder referenceFunder name
Histiocytosis Association
Histiocytosis Research Trust
1105891Bright Red (registered charity)
WT088555MAWellcome Trust
WT101155/Z/13/ZWellcome Trust