Toggle Main Menu Toggle Search

Open Access padlockePrints

Light and scanning electron microscopy of the same human metaphase chromosomes

Lookup NU author(s): Professor Christine Harrison FRCPath FMedSci


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


A technique has been developed to examine the same G-banded human metaphase chromosomes, first in the light microscope and then in the scanning electron microscope (SEM). A structural involvement in chromosome banding was confirmed by a positional correlation between the G-positive bands observed in the light microscope and the circumferential grooves between the quaternary coils of the metaphase chromosomes, observed in the SEM. In further support of this the regions between the grooves showed a positional relationship with the G-negative or reverse (R) bands. The examination of slightly extended metaphase chromosomes in the light microscope demonstrated that the G-banding pattern corresponded to that described by the Paris nomenclature for metaphase chromosomes. The arrangement of the circumferential grooves of the same chromosomes, observed in the SEM, was shown to relate to that described by the Paris nomenclature for prometaphase chromosomes. Therefore, using the SEM it is possible to demonstrate the details of prometaphase banding in metaphase chromosomes.

Publication metadata

Author(s): Harrison CJ, Jack EM, Allen TD, Harris R

Publication type: Article

Publication status: Published

Journal: Journal of Cell Science

Year: 1985

Volume: 77

Issue: 1

Pages: 143-153

Print publication date: 01/08/1985

ISSN (print): 0021-9533

ISSN (electronic): 1477-9137

Publisher: The Company of Biologists Ltd.


Notes: Journal Article Research Support, Non-U.S. Gov't England