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High-resolution scanning electron microscopy of human metaphase chromosomes

Lookup NU author(s): Professor Christine Harrison FRCPath FMedSci


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Human metaphase chromosomes, prepared for light microscopy were examined by scanning electron microscopy. Use of an osmium impregnation technique eliminated the need for sputter-coating and allowed high-resolution visualization of uncoated specimens. Chromosomes were of three-dimensional cylindrical profile, with well-defined chromatids and centromeres. Prior to Giemsa-banding a smooth surface morphology was observed. Relaxation of chromosome integrity by Giemsa-banding pretreatment allowed resolution of several orders of chromosome structure not previously demonstrated by scanning electron microscopy. The observed organization of the chromatin fibres allowed parallels to be drawn with the radial loop model of chromosome construction as described by Marsden and Laemmli.

Publication metadata

Author(s): Harrison CJ, Allen TD, Britch M, Harris R

Publication type: Article

Publication status: Published

Journal: Journal of Cell Science

Year: 1982

Volume: 56

Issue: 1

Pages: 409-422

Print publication date: 01/08/1982

ISSN (print): 0021-9533

ISSN (electronic): 1477-9137

Publisher: The Company of Biologists Ltd.


Notes: Journal Article Research Support, Non-U.S. Gov't England