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Structural Insights into Outer Membrane Permeability of Acinetobacter baumannii

Lookup NU author(s): Dr Michael Zahn, Dr Arnaud Basle, Professor Bert van den Berg

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Abstract

Bacterial resistance against antibiotics is an increasing global health problem. In Gram-negative bacteria the low permeability of the outer membrane (OM) is a major factor contributing to resistance, making it important to understand channel-mediated small-molecule passage of the OM. Acinetobacter baumannii has five Occ (OM carboxylate channel) proteins, which collectively are of major importance for the entry of small molecules. To improve our understanding of the OM permeability of A. baumannii, we present here the X-ray crystal structures of four Occ proteins, renamed OccAB1 to OccAB4. In addition we have carried out a biochemical and biophysical characterization using electrophysiology and liposome swelling experiments, providing information on substrate specificities. We identify OccAB1 as having the largest pore of the Occ proteins with corresponding high rates of small-molecule uptake, and we suggest that the future design of efficient antibiotics should focus on scaffolds that can permeate efficiently through the OccAB1 channel.

Publication metadata

Author(s): Zahn M, Bhamidimarri SP, Basle A, Winterhalter M, van den Berg B

Publication type: Article

Publication status: Published

Journal: Structure

Year: 2016

Volume: 24

Issue: 2

Pages: 221-231

Print publication date: 02/02/2016

Online publication date: 21/01/2016

Acceptance date: 16/12/2015

ISSN (print): 0969-2126

ISSN (electronic): 1878-4186

Publisher: Cell Press

URL: http://dx.doi.org/10.1016/j.str.2015.12.009

DOI: 10.1016/j.str.2015.12.009

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