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Lookup NU author(s): Emeritus Professor John Kirby,
Professor Simi Ali
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Chemokines have a range of functions, including the activation and promotion of the vectorial migration of leukocytes. They mediate their biological effects by binding to their cognate G-protein-coupled receptors. Upon activation of the heterotrimeric G proteins, the G alpha subunit exchanges GDP for GTP and dissociates from the receptor and from the G beta. subunits, and both G-protein complexes go on to activate other downstream signaling events. In addition, chemokines interact with cell-surface glycosaminoglycans (GAGs). This potential for binding GAG components of proteoglycans on the cell surface or within the extracellular matrix allows the formation of the stable chemokine gradients necessary for leukocyte chemotaxis. In this chapter, we describe techniques for studying chemotaxis both in vivo and in vitro, as well as the creation of chemokine receptor-expressing cell lines, in order to examine this process in isolation.
Author(s): del Barrio ID, Kirby J, Ali S
Publication type: Review
Publication status: Published
Journal: Methods in Enzymolgy
Online publication date: 14/11/2015
Acceptance date: 01/01/1900
ISSN (print): 0076-6879
Publisher: ELSEVIER ACADEMIC PRESS INC