Browse by author
Lookup NU author(s): Professor James Allan
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Background: Rare variants (<1%) likely contribute significantly to risk for common diseases such as inflammatory bowel disease (IBD) in specific patient subsets, such as those with high familiality. They are, however, extraordinarily challenging to identify.Methods: To discover candidate rare variants associated with IBD, we performed whole-exome sequencing on 6 members of a pediatric-onset IBD family with multiple affected individuals. To determine whether the variants discovered in this family are also associated with nonfamilial IBD, we investigated their influence on disease in 2 large case-control (CC) series.Results: We identified 2 rare variants, rs142430606 and rs200958270, both in the established IBD-susceptibility gene IL17REL, carried by all 4 affected family members and their obligate carrier parents. We then demonstrated that both variants are associated with sporadic ulcerative colitis (UC) in 2 independent data sets. For UC in CC 1: rs142430606 (odds ratio [OR] = 2.99, P-adj = 0.028; minor allele frequency [MAF](cases) = 0.0063, MAF(controls) = 0.0021); rs200958270 (OR = 2.61, P-adj = 0.082; MAF(cases) = 0.0045, MAF(controls) = 0.0017). For UC in CC 2: rs142430606 (OR = 1.94, P = 0.0056; MAF(cases) = 0.0071, MAF(controls) = 0.0045); rs200958270 (OR = 2.08, P = 0.0028; MAF(cases) = 0.0071, MAF(controls) = 0.0042).Conclusions: We discover in a family and replicate in 2 CC data sets 2 rare susceptibility variants for IBD, both in IL17REL. Our results illustrate that whole-exome sequencing performed on disease-enriched families to guide association testing can be an efficient strategy for the discovery of rare disease-associated variants. We speculate that rare variants identified in families and confirmed in the general population may be important modifiers of disease risk for patients with a family history, and that genetic testing of these variants may be warranted in this patient subset.
Author(s): Sasaki MM, Skol AD, Hungate EA, Bao RY, Huang L, Kahn SA, Allan JM, Brant SR, McGovern DPB, Peter I, Silverberg MS, Cho JH, Kirschner BS, Onel K
Publication type: Article
Publication status: Published
Journal: Inflammatory Bowel Diseases
Year: 2016
Volume: 22
Issue: 1
Pages: 20-27
Print publication date: 01/01/2016
Acceptance date: 01/01/1900
ISSN (print): 1078-0998
ISSN (electronic): 1536-4844
Publisher: Lippincott Williams & Wilkins, Ltd.
URL: http://dx.doi.org/10.1097/MIB.0000000000000610
DOI: 10.1097/MIB.0000000000000610
Altmetrics provided by Altmetric
