Toggle Main Menu Toggle Search

Open Access padlockePrints

Transcytosis of the polymeric Ig receptor requires phosphorylation of serine 664 in the absence but not the presence of dimeric IgA

Lookup NU author(s): Professor Robert HirtORCiD


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


MDCK cells expressing the polymeric immunoglobulin (poly-Ig) receptor, cocultured with IgA-producing hybridoma cells, transported dimeric IgA (dIgA) from the basolateral into the lumenal compartment, where it was recovered as secretory component-dIgA complexes. The tail of the receptor was phosphorylated on serines 664 and 726. Each serine was mutated to alanine. Appearance of A726 receptor at the basolateral surface was reduced approximately 5-fold. This was accompanied by a approximately 5-fold reduction in dIgA transcytosis. Basolateral delivery of receptor was not affected by mutation A664, and in the absence of dIgA, the receptor accumulated in recycling basolateral endosomes. In coculture, however, dIgA transcytosis by A664 receptor was normal. Thus, entry of receptor into the transcytotic pathway requires Ser-664 phosphorylation only in the absence of dIgA.

Publication metadata

Author(s): Hirt RP; Hughes GJ; Frutiger S; Michetti P; Perregaux C; Poulain-Godefroy O; Jeanguenat N; Neutra MR; Kraehenbuhl JP

Publication type: Article

Publication status: Published

Journal: Cell

Year: 1993

Volume: 74

Issue: 2

Pages: 245-255

Print publication date: 30/07/1993

ISSN (print): 0092-8674

ISSN (electronic): 1097-4172

Publisher: Cell Press


DOI: 10.1016/0092-8674(93)90416-N


Altmetrics provided by Altmetric