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Sharing Structure and Function in Biological Design with SBOL 2.0

Lookup NU author(s): Dr Goksel Misirli, Dr Curtis Madsen, Professor Anil Wipat

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Abstract

The Synthetic Biology Open Language (SBOL) is a standard that enables collaborative engineering of biological systems across different institutions and tools. SBOL is developed through careful consideration of recent synthetic biology trends, real use cases, and consensus among leading researchers in the field and members of commercial biotechnology enterprises. We demonstrate and discuss how a set of SBOL-enabled software tools can form an integrated, cross-organizational workflow to recapitulate the design of one of the largest published genetic circuits to date, a 4-input AND sensor. This design encompasses the structural components of the system, such as its DNA, RNA, small molecules, and proteins, as well as the interactions between these components that determine the system's behavior/function. The demonstrated workflow and resulting circuit design illustrate the utility of SBOL 2.0 in automating the exchange of structural and functional specifications for genetic parts, devices, and the biological systems in which they operate.

Publication metadata

Author(s): Roehner N, Beal J, Clancy K, Bartley B, Misirli G, Grunberg R, Oberortner E, Pocock M, Bissell M, Madsen C, Nguyen T, Zhang M, Zhang Z, Zundel Z, Densmore D, Gennari JH, Wipat A, Sauro HM, Myers CJ

Publication type: Article

Publication status: Published

Journal: ACS Synthetic Biology

Year: 2016

Volume: 5

Issue: 6

Pages: 498-506

Print publication date: 01/06/2016

Online publication date: 25/04/2016

Acceptance date: 31/10/2015

ISSN (electronic): 2161-5063

Publisher: American Chemical Society

URL: http://dx.doi.org/10.1021/acssynbio.5b00215

DOI: 10.1021/acssynbio.5b00215

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