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Discovery of Diverse Small-Molecule Inhibitors of Mammalian Sterile20-like Kinase 3 (MST3)

Lookup NU author(s): Dr Mathew Martin

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Abstract

Increasing evidence suggests key roles for members of the mammalian Sterile20-like (MST) family of kinases in many aspects of biology. MST3 is a member of the STRIPAK complex, the deregulation of which has recently been associated with cancer cell migration and metastasis. Targeting MST3 with small-molecule inhibitors may be beneficial for the treatment of certain cancers, but little information exists on the potential of kinase inhibitor scaffolds to engage with MST3. In this study we screened MST3 against a library of 277 kinase inhibitors using differential scanning fluorimetry and confirmed 14 previously unknown MST3 inhibitors by X-ray crystallography. These compounds, of which eight are in clinical trials or FDA approved, comprise nine distinct chemical scaffolds that inhibit MST3 enzymatic activity with IC50 values between 0.003 and 23 mu m. The structure-activity relationships explain the differential inhibitory activity of these compounds against MST3 and the structural basis for high binding potential, the information of which may serve as a framework for the rational design of MST3-selective inhibitors as potential therapeutics and to interrogate the function of this enzyme in diseased cells.


Publication metadata

Author(s): Olesen SH, Zhu JY, Martin MP, Schonbrunn E

Publication type: Article

Publication status: Published

Journal: ChemMedChem

Year: 2016

Volume: 11

Issue: 11

Pages: 1137-1144

Print publication date: 01/06/2016

Online publication date: 02/05/2016

Acceptance date: 02/04/2016

ISSN (print): 1860-7179

ISSN (electronic): 1860-7187

Publisher: Wiley - VCH Verlag GmbH & Co. KGaA

URL: http://dx.doi.org/10.1002/cmdc.201600115

DOI: 10.1002/cmdc.201600115


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