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An iTRAQ characterisation of the role of TolC during electron transfer from Shewanella oneidensis MR-1

Lookup NU author(s): Emeritus Professor Pete Wright


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Anodophilic bacteria have the ability to generate electricity in microbial fuel cells (MFCs) by extracellular electron transfer to the anode. We investigated the anode-specific responses of Shewanella oneidensis MR-1, an exoelectroactive Gammaproteobacterium, using for the first time iTRAQ and 2D-LC MS/MS driven membrane proteomics to compare protein abundances in S. oneidensis when generating power in MFCs, and growing in a continuous culture. The regulated dataset produced was enriched in membrane proteins. Proteins shown to be more abundant in anaerobic electroactive anodic cells included efflux pump TolC and an uncharacterised tetratricopeptide repeat (TPR) protein, whilst the TonB2 system and associated uncharacterised proteins such as TtpC2 and DUF3450 were more abundant in microaerobic planktonic cells. In order to validate the iTRAQ data, the functional role for TolC was examined using a TolC knockout mutant of S. oneidensis. Possible roles for the uncharacterised proteins were identified using comparative bioinformatics. We demonstrate that employing an insoluble extracellular electron acceptor requires multiple proteins involved in cell surface properties. All MS and processed data are available via ProteomeXchange with identifier PXD004090.

Publication metadata

Author(s): Fowler GJS, Pereira-Medrano AG, Jaffe S, Pasternak G, Pham TK, Ledezma P, Hall STE, Ieropoulos IA, Wright PC

Publication type: Article

Publication status: Published

Journal: Proteomics

Year: 2016

Volume: 16

Issue: 21

Pages: 2764-2775

Print publication date: 01/11/2016

Online publication date: 18/10/2016

Acceptance date: 02/09/2016

ISSN (print): 1615-9853

ISSN (electronic): 1615-9861

Publisher: Wiley-Blackwell Publishing Ltd.


DOI: 10.1002/pmic.201500538


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Funder referenceFunder name
University of Sheffield
GR/113559/11EPSRC under the ChELSI Initiative
X/004170/19United Kingdom's Engineering and Physical Science Research Council (EPSRC)