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Lookup NU author(s): Emeritus Professor Pete Wright
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Anodophilic bacteria have the ability to generate electricity in microbial fuel cells (MFCs) by extracellular electron transfer to the anode. We investigated the anode-specific responses of Shewanella oneidensis MR-1, an exoelectroactive Gammaproteobacterium, using for the first time iTRAQ and 2D-LC MS/MS driven membrane proteomics to compare protein abundances in S. oneidensis when generating power in MFCs, and growing in a continuous culture. The regulated dataset produced was enriched in membrane proteins. Proteins shown to be more abundant in anaerobic electroactive anodic cells included efflux pump TolC and an uncharacterised tetratricopeptide repeat (TPR) protein, whilst the TonB2 system and associated uncharacterised proteins such as TtpC2 and DUF3450 were more abundant in microaerobic planktonic cells. In order to validate the iTRAQ data, the functional role for TolC was examined using a TolC knockout mutant of S. oneidensis. Possible roles for the uncharacterised proteins were identified using comparative bioinformatics. We demonstrate that employing an insoluble extracellular electron acceptor requires multiple proteins involved in cell surface properties. All MS and processed data are available via ProteomeXchange with identifier PXD004090.
Author(s): Fowler GJS, Pereira-Medrano AG, Jaffe S, Pasternak G, Pham TK, Ledezma P, Hall STE, Ieropoulos IA, Wright PC
Publication type: Article
Publication status: Published
Journal: Proteomics
Year: 2016
Volume: 16
Issue: 21
Pages: 2764-2775
Print publication date: 01/11/2016
Online publication date: 18/10/2016
Acceptance date: 02/09/2016
ISSN (print): 1615-9853
ISSN (electronic): 1615-9861
Publisher: Wiley-Blackwell Publishing Ltd.
URL: http://dx.doi.org/10.1002/pmic.201500538
DOI: 10.1002/pmic.201500538
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