Toggle Main Menu Toggle Search

Open Access padlockePrints

A Novel Carbohydrate-binding Module from Sugar Cane Soil Metagenome Featuring Unique Structural and Carbohydrate Affinity Properties

Lookup NU author(s): Emeritus Professor Harry Gilbert


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Carbohydrate-binding modules (CBMs) are appended to glycoside hydrolases and can contribute to the degradation of complex recalcitrant substrates such as the plant cell wall. For application in bioethanol production, novel enzymes with high catalytic activity against recalcitrant lignocellulosic material are being explored and developed. In this work, we report the functional and structural study of CBM_E1, which was discovered through a metagenomics approach and is the founding member of a novel CBM family, CBM81. CBM_E1, which is linked to an endoglucanase, displayed affinity for mixed linked 1,3-1,4-glucans, xyloglucan, Avicel, and cellooligosaccharides. The crystal structure of CBM_E1 in complex with cellopentaose displayed a canonical -sandwich fold comprising two -sheets. The planar ligand binding site, observed in a parallel orientation with the -strands, is a typical feature of type A CBMs, although the expected affinity for bacterial crystalline cellulose was not detected. Conversely, the binding to soluble glucans was enthalpically driven, which is typical of type B modules. These unique properties of CBM_E1 are at the interface between type A and type B CBMs.

Publication metadata

Author(s): Campos BM, Liberato MV, Alvarez TM, Zanphorlin LM, Ematsu GC, Barud H, Polikarpov I, Ruller R, Gilbert HJ, Zeri ACD, Squina FM

Publication type: Article

Publication status: Published

Journal: Journal of Biological Chemistry

Year: 2016

Volume: 291

Issue: 45

Pages: 23734-23743

Print publication date: 04/11/2016

Online publication date: 12/09/2016

Acceptance date: 02/04/2016

ISSN (print): 0021-9258

ISSN (electronic): 1083-351X

Publisher: American Society for Biochemistry and Molecular Biology, Inc.


DOI: 10.1074/jbc.M116.744383


Altmetrics provided by Altmetric


Funder referenceFunder name
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil
2013/06336-0Fundacao de Amparo a Pesquisa do Estado de Sao Paulo Process
2008/58037-9Fundacao de Amparo a Pesquisa do Estado de Sao Paulo Process
2010/11469-1Fundacao de Amparo a Pesquisa do Estado de Sao Paulo Process
2014/04105-4Fundacao de Amparo a Pesquisa do Estado de Sao Paulo Process