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Development of multi-substituted hydroxyapatite nanopowders as biomedical materials for bone tissue engineering applications

Lookup NU author(s): Dr Yanny Baba Ismail, Dr Oana Bretcanu, Professor Kenneth Dalgarno

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Abstract

Ionic substitutions have been proposed as a tool to control the functional behavior of synthetic hydroxyapatite (HA), particularly for Bone Tissue Engineering (BTE) applications. The effect of simultaneous substitution of different levels of carbonate (CO₃) and silicon (Si) ions in the HA lattice was investigated. Furthermore, human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on multi-substituted HA (SiCHA) to determine if biomimetic chemical compositions were osteoconductive. Of the four different compositions investigates, SiCHA-1 (0.58wt% Si) and SiCHA-2 (0.45wt% Si) showed missing bands for CO₃ and Si using FTIR analysis, indicating competition for occupation of the phosphate site in the HA lattice. 500°C was considered the most favourable calcination temperature as: (i) the powders produced possessed a similar amount of CO₃ (2-8wt%) and Si (<1.0wt%) as present in native bone; and (ii) there was a minimal loss of CO₃ and Si from the HA structure to the surroundings during calcination. Higher Si content in SiCHA-1 led to lower cell viability and at most hindered proliferation, but no toxicity effect occurred. While, lower Si content in SiCHA-2 showed the highest ALP/DNA ratio after 21 days culture with hMSCs, indicating that the powder may stimulate osteogenic behaviour to a greater extent than other powders.

Publication metadata

Author(s): Baba Ismail YM, Wimpenny I, Bretcanu O, Dalgarno K, El Haj AJ

Publication type: Article

Publication status: Published

Journal: Journal of Biomedical Materials Research Part A

Year: 2017

Volume: 105

Issue: 6

Pages: 1775-1785

Print publication date: 01/06/2017

Online publication date: 15/02/2017

Acceptance date: 10/02/2017

Date deposited: 16/02/2017

ISSN (print): 1549-3296

ISSN (electronic): 1552-4965

Publisher: John Wiley & Sons, Inc.

URL: http://dx.doi.org/10.1002/jbm.a.36038

DOI: 10.1002/jbm.a.36038

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