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Development of multi-substituted hydroxyapatite nanopowders as biomedical materials for bone tissue engineering applications

Lookup NU author(s): Dr Yanny Baba Ismail, Dr Oana Bretcanu, Professor Kenneth Dalgarno



Ionic substitutions have been proposed as a tool to control the functional behavior of synthetic hydroxyapatite (HA), particularly for Bone Tissue Engineering (BTE) applications. The effect of simultaneous substitution of different levels of carbonate (CO3) and silicon (Si) ions in the HA lattice was investigated. Furthermore, human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured on multi-substituted HA (SiCHA) to determine if biomimetic chemical compositions were osteoconductive. Of the four different compositions investigates, SiCHA-1 (0.58wt% Si) and SiCHA-2 (0.45wt% Si) showed missing bands for CO3 and Si using FTIR analysis, indicating competition for occupation of the phosphate site in the HA lattice. 500°C was considered the most favourable calcination temperature as: (i) the powders produced possessed a similar amount of CO3 (2-8wt%) and Si (<1.0wt%) as present in native bone; and (ii) there was a minimal loss of CO3 and Si from the HA structure to the surroundings during calcination. Higher Si content in SiCHA-1 led to lower cell viability and at most hindered proliferation, but no toxicity effect occurred. While, lower Si content in SiCHA-2 showed the highest ALP/DNA ratio after 21 days culture with hMSCs, indicating that the powder may stimulate osteogenic behaviour to a greater extent than other powders.

Publication metadata

Author(s): Baba Ismail YM, Wimpenny I, Bretcanu O, Dalgarno K, El Haj AJ

Publication type: Article

Publication status: Published

Journal: Journal of Biomedical Materials Research Part A

Year: 2017

Volume: 105

Issue: 6

Pages: 1775-1785

Print publication date: 01/06/2017

Online publication date: 15/02/2017

Acceptance date: 10/02/2017

Date deposited: 16/02/2017

ISSN (print): 1549-3296

ISSN (electronic): 1552-4965

Publisher: John Wiley & Sons, Inc.


DOI: 10.1002/jbm.a.36038


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