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A cell line from Wilms' tumour with deletion in short arm of chromosome II

Lookup NU author(s): Professor Christine Harrison FRCPath FMedSci


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A cell line (T3/73) from a Wilms' tumour has been established from a 9 month-old boy with aniridia. The tumour was removed in 1973. On histological examination a diagnosis of Wilms' tumour was made which showed undifferentiated areas, marked tubule formation and abundant striped muscle fibres. The tumour cells, which are fusiform, grew rapidly in culture without the addition of growth factors, and have undergone over 100 passages. Approximately 95% and 5% were positive for desmin and cytokeratin, respectively. The cell doubling time was 28 hr. Cytogenetic studies revealed a karyotype of 46,XY,del(11) (p12::p14). Although the cells stained very intensely with a monoclonal antibody that detects oncogene ras p 21 antigen, Southern blot analysis using c-Ha-ras as a probe failed to reveal an obvious deletion or amplification of either Ha-ras allele.

Publication metadata

Author(s): Kumar S, Harrison CJ, Heighway J, Marsden HB, West DC, Jones PM

Publication type: Article

Publication status: Published

Journal: International Journal of Cancer

Year: 1987

Volume: 40

Issue: 4

Pages: 499-504

Print publication date: 15/10/1987

ISSN (print): 0020-7136

ISSN (electronic): 1097-0215

Publisher: John Wiley & Sons, Inc.


DOI: 10.1002/ijc.2910400412

Notes: Case Reports Journal Article United states


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