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Detecting senescence: a new method for an old pigment

Lookup NU author(s): Hanna Salmonowicz, Dr Joao Passos

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2017 The Anatomical Society and John Wiley & Sons Ltd.Cellular senescence is a state of irreversible cell cycle arrest induced by different types of cellular stresses. The field of senescence has made significant advances in the understanding of many of the mechanisms governing this phenomenon; however, a universal biomarker that unambiguously distinguishes senescent from proliferating cells has not been found. In this issue of Aging Cell, Evangelou and colleagues developed a sensitive method for identification of senescent cells in different types of biological material based on the detection of lipofuscin using an analogue of Sudan Black B (SBB) histochemical dye coupled with biotin, which they named GL13. The authors propose that this method is more sensitive and versatile than using SBB alone. Lipofuscin, a nondegradable oxidation product of lipids, proteins and metals, is found in senescent cells. Detection of lipofuscin using GL13 staining may be a more feasible method than others currently used for identification of senescent cells both in cell culture and tissues.


Publication metadata

Author(s): Salmonowicz H, Passos JF

Publication type: Article

Publication status: Published

Journal: Aging Cell

Year: 2017

Volume: 16

Issue: 3

Pages: 432-434

Print publication date: 01/06/2017

Online publication date: 09/02/2017

Acceptance date: 17/01/2017

Date deposited: 12/04/2017

ISSN (print): 1474-9718

ISSN (electronic): 1474-9726

Publisher: Wiley-Blackwell

URL: https://doi.org/10.1111/acel.12580

DOI: 10.1111/acel.12580


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