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Real-Time LAMP for Chalara fraxinea Diagnosis

Lookup NU author(s): Professor Neil Boonham

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Abstract

© Springer Science+Business Media New York 2015Chalara fraxinea is the causal agent of ash dieback, a disease affecting Fraxinus excelsior and F. angustifolia across Europe. Loop-mediated isothermal amplifi cation (LAMP) is a rapid, DNA-based method which can be used for specifi c detection of plant pathogens in infected material. The combination of a rapid LAMP assay for C. fraxinea with a simple sample preparation method in a user-friendly kit format raises the potential for testing to be carried out away from conventional laboratory facilities, to expedite measure to manage this damaging disease.

Publication metadata

Author(s): Tomlinson J, Boonham N

Editor(s): Christophe Lacomme

Publication type: Book Chapter

Publication status: Published

Book Title: Plant Pathology: Techniques and Protocols

Year: 2015

Volume: 1302

Pages: 75-83

Online publication date: 01/01/2015

Acceptance date: 01/01/1900

Series Title: Methods in Molecular Biology

Publisher: Humana Press Inc.

URL: https://doi.org/10.1007/978-1-4939-2620-6_6

DOI: 10.1007/978-1-4939-2620-6_6

PubMed id: 25981247

Library holdings: Search Newcastle University Library for this item

ISBN: 9781493926190

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