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Lookup NU author(s): Professor Neil Boonham
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
© 2014 The Authors. Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology. In Europe the most devastating phytoplasma associated with grapevine yellows (GY) diseases is a quarantine pest, flavescence dorée (FDp), from the 16SrV taxonomic group. The on-site detection of FDp with an affordable device would contribute to faster and more efficient decisions on the control measures for FDp. Therefore, a real-time isothermal LAMP assay for detection of FDp was validated according to the EPPO standards and MIQE guidelines. The LAMP assay was shown to be specific and extremely sensitive, because it detected FDp in all leaf samples that were determined to be FDp infected using quantitative real-time PCR. The whole procedure of sample preparation and testing was designed and optimized for on-site detection and can be completed in one hour. The homogenization procedure of the grapevine samples (leaf vein, flower or berry) was optimized to allow direct testing of crude homogenates with the LAMP assay, without the need for DNA extraction, and was shown to be extremely sensitive.
Author(s): Kogovsek P, Hodgetts J, Hall J, Prezelj N, Nikolic P, Mehle N, Lenarcic R, Rotter A, Dickinson M, Boonham N, Dermastia M, Ravnikar M
Publication type: Article
Publication status: Published
Journal: Plant Pathology
Year: 2015
Volume: 64
Issue: 2
Pages: 286-296
Print publication date: 01/04/2015
Online publication date: 22/07/2014
Acceptance date: 21/06/2014
Date deposited: 28/06/2017
ISSN (print): 0032-0862
ISSN (electronic): 1365-3059
Publisher: Wiley-Blackwell
URL: https://doi.org/10.1111/ppa.12266
DOI: 10.1111/ppa.12266
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