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Risk assessment procedure for Cucumber Vein Yellowing Virus (CVYV) and Cucurbit Yellow Stunting Disorder Virus (CYSDV) using a real time RT-PCR assay - study of virus levels in the vector Bemisia tabaci

Lookup NU author(s): Professor Giles Budge, Professor Neil Boonham


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Cucumber vein yellowing virus (CVYV) (genus Ipomovirus, family Potyviridae) and Cucurbit yellow stunting disorder virus (CYSDV) (genus Crinivirus, family Closteroviridae) are included in the EPPO A2 Action List of pathogens and have become a limiting factor of cucumber production in the southeast of Spain. Due to the importance of these two pathogens and the need to have robust tools that can be used in risk assessment procedures to predict future epidemics, a real-time RT-PCR method based on TaqMan® chemistry has been developed. This method is fast, sensitive, specific and easy to automate in plant health laboratories.

Publication metadata

Author(s): Gil-Salas FM, Morris J, Cuadrado IM, Budge G, Colyer A, Boonham N, Janssen D

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: International Symposium on Vegetable Production, Quality and Process Standardization in Chain: a Worldwide Perspective

Year of Conference: 2008

Pages: 59-62

Print publication date: 01/04/2012

ISSN: 0567-7572

Publisher: International Society for Horticultural Science


DOI: 10.17660/ActaHortic.2012.944.7

Library holdings: Search Newcastle University Library for this item

Series Title: Acta Horticulturae

ISBN: 9789066051874