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Detection of honey bee (Apis mellifera) viruses with an oligonucleotide microarray

Lookup NU author(s): Professor Giles Budge, Professor Neil Boonham

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Abstract

In recent years, declines in honey bee (Apis mellifera L.) colonies have been observed to varying degrees worldwide with the worst losses in the USA being termed Colony Collapse Disorder (CCD). Pathogen load and the prevalence of honey bee viruses have been implicated in these losses and many diseased hives have multiple viruses present. We have designed and tested an oligonucleotide microarray which enables the simultaneous detection of nine honey bee viruses: Acute bee paralysis virus, Black queen cell virus, Chronic bee paralysis virus, Deformed wing virus, Kashmir bee virus, Sacbrood virus, Israel acute paralysis virus, Varroa destructor virus 1 and Slow paralysis virus. The microarray can be used to robustly diagnose nine viruses in one test. © 2011.

Publication metadata

Author(s): Glover RH, Adams IP, Budge G, Wilkins S, Boonham N

Publication type: Article

Publication status: Published

Journal: Journal of Invertebrate Pathology

Year: 2011

Volume: 107

Issue: 3

Pages: 216-219

Print publication date: 01/07/2011

Online publication date: 16/03/2011

ISSN (print): 0022-2011

ISSN (electronic): 1096-0805

Publisher: Academic Press

URL: https://doi.org/10.1016/j.jip.2011.03.004

DOI: 10.1016/j.jip.2011.03.004

PubMed id: 21419132

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