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Development of real-time RT-PCR assays for the detection of Cucumber vein yellowing virus (CVYV) and Cucurbit yellow stunting disorder virus (CYSDV) in the whitefly vector Bemisia tabaci

Lookup NU author(s): Professor Giles Budge, Professor Neil Boonham


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Reverse transcription followed by real-time PCR assays based on TaqMan® chemistry have been developed for the detection and quantification of Cucumber vein yellowing virus (CVYV) and Cucurbit yellow stunting disorder virus (CYSDV) in individual adults of the whitefly vector Bemisia tabaci. The method includes an internal control for the detection of a gene from B. tabaci to compensate for variations in extraction efficiency. The assays designed were used to estimate proportions of viruliferous whiteflies collected from commercial greenhouse-grown crops in Spain. In a significant number of whiteflies, both viruses were detected and their amounts were estimated. The assays could be used to assist risk assessment of CVYV and CYSDV which constitute limiting factors in cucurbit crops. They are also suited to investigating the epidemiology and plant-virus-vector relationships in these diseases. © 2007 Elsevier B.V. All rights reserved.

Publication metadata

Author(s): Gil-Salas FM, Morris J, Colyer A, Budge G, Boonham N, Cuadrado IM, Janssen D

Publication type: Article

Publication status: Published

Journal: Journal of Virological Methods

Year: 2007

Volume: 146

Issue: 1-2

Pages: 45-51

Print publication date: 01/12/2007

Online publication date: 31/05/2007

ISSN (print): 0166-0934

ISSN (electronic): 1879-0984

Publisher: Elsevier BV


DOI: 10.1016/j.jviromet.2007.05.032

PubMed id: 17624449


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