Toggle Main Menu Toggle Search

Open Access padlockePrints

Multiplex High-Throughput Targeted Proteomic Assay to Identify Induced Pluripotent Stem Cells

Lookup NU author(s): Professor Majlinda Lako, Professor Lyle Armstrong

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

© 2017 American Chemical Society. Induced pluripotent stem cells have great potential as a human model system in regenerative medicine, disease modeling, and drug screening. However, their use in medical research is hampered by laborious reprogramming procedures that yield low numbers of induced pluripotent stem cells. For further applications in research, only the best, competent clones should be used. The standard assays for pluripotency are based on genomic approaches, which take up to 1 week to perform and incur significant cost. Therefore, there is a need for a rapid and cost-effective assay able to distinguish between pluripotent and nonpluripotent cells. Here, we describe a novel multiplexed, high-throughput, and sensitive peptide-based multiple reaction monitoring mass spectrometry assay, allowing for the identification and absolute quantitation of multiple core transcription factors and pluripotency markers. This assay provides simpler and high-throughput classification into either pluripotent or nonpluripotent cells in 7 min analysis while being more cost-effective than conventional genomic tests.


Publication metadata

Author(s): Baud A, Wessely F, Mazzacuva F, McCormick J, Camuzeaux S, Heywood WE, Little D, Vowles J, Tuefferd M, Mosaku O, Lako M, Armstrong L, Webber C, Cader MZ, Peeters P, Gissen P, Cowley SA, Mills K

Publication type: Article

Publication status: Published

Journal: Analytical Chemistry

Year: 2017

Volume: 89

Issue: 4

Pages: 2440-2448

Online publication date: 11/01/2017

Acceptance date: 11/01/2017

ISSN (print): 0003-2700

ISSN (electronic): 1520-6882

Publisher: American Chemical Society

URL: https://doi.org/10.1021/acs.analchem.6b04368

DOI: 10.1021/acs.analchem.6b04368


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share