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Lookup NU author(s): Professor Tiago OuteiroORCiD
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© The Author 2017. Published by Oxford University Press. All rights reserved. Huntington's disease is neurodegenerative disorder caused by a polyglutamine expansion in the N-terminal region of the huntingtin protein (N17). Here, we analysed the relative contribution of each phosphorylatable residue in the N17 region (T3, S13 and S16) towards huntingtin exon 1 (HTTex1) oligomerization, aggregation and toxicity in human cells and Drosophila neurons.We used bimolecular fluorescence complementation to show that expression of single phosphomimic mutations completely abolished HTTex1 aggregation in human cells. In Drosophila, mimicking phosphorylation at T3 decreased HTTex1 aggregation both in larvae and adult flies. Interestingly, pharmacological or genetic inhibition of protein phosphatase 1 (PP1) prevented HTTex1 aggregation in both human cells and Drosophila while increasing neurotoxicity in flies. Our findings suggest that PP1 modulates HTTex1 aggregation by regulating phosphorylation on T3. In summary, our study suggests that modulation of HTTex1 single phosphorylation events by PP1 could constitute an efficient and direct molecular target for therapeutic interventions in Huntington's disease.
Author(s): Branco-Santos J, Herrera F, Pocas GM, Pires-Afonso Y, Giorgini F, Domingos PM, Outeiro TF
Publication type: Article
Publication status: Published
Journal: Human Molecular Genetics
Year: 2017
Volume: 26
Issue: 19
Pages: 3763-3775
Print publication date: 01/10/2017
Online publication date: 07/07/2017
Acceptance date: 30/06/2017
ISSN (print): 0964-6906
ISSN (electronic): 1460-2083
Publisher: Oxford University Press
URL: https://doi.org/10.1093/hmg/ddx260
DOI: 10.1093/hmg/ddx260
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