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Lookup NU author(s): Professor Tiago OuteiroORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
© 2014 The Authors. Published by Elsevier Ltd. Alpha-synuclein (aSyn) is a key player in Parkinson's disease. Genetically engineered yeast cells producing aSyn fused with GFP (aSyn-GFP) have been used to study this protein. In this work, we present a microfluidic platform with integrated photosensors that captures single yeast cells in arrays of hydrodynamic traps and exposes them to a chemical gradient of precise composition. This platform enables the study of the effects of aSyn expression level and aggregation in genetically modified yeast cells by chemical stimulation. The photosensors allow the detection of cells in the traps by measuring the variations in light transmission or of the fluorescence produced by aSyn-GFP for real-time signal acquisition.
Author(s): Fernandes JTS, Tenreiro S, Pedrosa CR, Gameiro A, Chu V, Outeiro TF, Conde JP
Publication type: Article
Publication status: Published
Journal: Procedia Engineering
Online publication date: 31/12/2014
Acceptance date: 01/01/1900
Date deposited: 19/12/2017
ISSN (print): 1877-7058
Publisher: Elsevier Ltd
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