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Assessing the subcellular dynamics of alpha-synuclein using photoactivation microscopy

Lookup NU author(s): Professor Tiago OuteiroORCiD

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Abstract

Alpha-synuclein (aSyn) is implicated in Parkinson's disease and several other neurodegenerative disorders. To date, the function and intracellular dynamics of aSyn are still unclear. Here, we tracked the dynamics of aSyn using photoactivatable green fluorescent protein as a reporter. We found that the availability of the aSyn N terminus modulates its shuttling into the nucleus. Interestingly, familial aSyn mutations altered the dynamics at which the protein distributes throughout the cell. Both the A30P and A53T aSyn mutations increase the speed at which the protein moves between the nucleus and cytoplasm, respectively. We also found that specific kinases potentiate the shuttling of aSyn between nucleus and cytoplasm. A mutant aSyn form that blocks S129 phos-phorylation, S129A, results in the formation of cytoplasmic inclusions, suggesting phosphorylation modulates aggregation in addition to modulating aSyn intracellular dynamics. Finally, we found that the molecular chaperone HSP70 accelerates the entry of aSyn into the nuclear compartment. © The Author(s) 2013.

Publication metadata

Author(s): Goncalves S, Outeiro TF

Publication type: Article

Publication status: Published

Journal: Molecular Neurobiology

Year: 2013

Volume: 47

Issue: 3

Pages: 1081-1092

Print publication date: 01/06/2013

Online publication date: 08/02/2013

ISSN (print): 0893-7648

ISSN (electronic): 1559-1182

Publisher: Springer

URL: https://doi.org/10.1007/s12035-013-8406-x

DOI: 10.1007/s12035-013-8406-x

PubMed id: 23389286

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