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Lookup NU author(s): Professor Anne Dickinson,
Dr Xiao Wang,
Dr Shaheda Ahmed
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© Springer International Publishing AG 2017. A human in vitro skin explant test was developed by Alcyomics for the prediction of adverse immune reactions and assessment of relative potency. The skin explant test has a unique readout of histological damage, which ranges in severity from grade 0 and I (negative reaction) to grades II, III and IV damage (positive reaction) in human full-thickness skin. The test has been assessed for predicting responses to sensitizers and non-sensitizers, originally tested in the mouse local lymph node assay (LLNA). Results compared with the LLNA gave 95% specificity, 95% sensitivity and 95% concordance with a correlation coefficient of 0.9. Similar specificity and sensitivity were achieved for comparison of results to published human data with a correlation coefficient of 0.91. The test can also identify chemicals mis-classified in the mouse local lymph node assay (LLNA). Results of the skin test correlate with high or low T cell proliferation and IFNγ production, and these assays can be used as in vitro screening tests for compounds. Collectively, the human in vitro skin explant test can provide a robust, reliable and novel approach for characterization of sensitizing activity as a first step in the risk assessment process.
Author(s): Dickinson A, Nong Wang X, Ahmed S
Publication type: Book Chapter
Publication status: Published
Book Title: Alternatives for Dermal Toxicity Testing
Online publication date: 19/06/2017
Acceptance date: 02/04/2016
Publisher: Springer International Publishing
Library holdings: Search Newcastle University Library for this item