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Insight into Metal Removal from Peptides that Sequester Copper for Methane Oxidation

Lookup NU author(s): Dr Arnaud Basle, Dr Abdelnasser El Ghazouani, Jaeick Lee, Professor Christopher Dennison



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Methanobactins (Mbns) are modified peptides that sequester copper (Cu) methanotrophs use to oxidize methane. Limited structural information is available for this class of natural products, as is an understanding of how cells are able to utilize Mbn-bound Cu. The crystal structure of Methylosinus sporium NR3K CuI-Mbn provides further information about the structural diversity of Mbns and the first insight into their Cu-release mechanism. Nitrogen ligands from oxazolone and pyrazinediol rings chelate CuI along with adjacent coordinating sulfurs from thioamides. In vitro solution data are consistent with a CuI-Mbn monomer as found for previously characterized Mbns. In the crystal structure, the N-terminal region has undergone a conformational change allowing the formation of a CuI 2-Mbn2 dimer with CuI sites bound by chelating units from adjacent chains. Such a structural alteration will facilitate CuI release from Mbns.

Publication metadata

Author(s): Baslé A, El Ghazouani A, Lee J, Dennison C

Publication type: Article

Publication status: Published

Journal: Chemistry - A European Journal

Year: 2018

Volume: 24

Issue: 18

Pages: 4515-4518

Print publication date: 26/03/2018

Online publication date: 24/01/2018

Acceptance date: 20/12/2017

Date deposited: 24/04/2018

ISSN (print): 0947-6539

ISSN (electronic): 1521-3765

Publisher: Wiley-VCH Verlag


DOI: 10.1002/chem.201706035


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Funder referenceFunder name
NE/F00608X/1Natural Environment Research Council (NERC)