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Lookup NU author(s): Dr Sara Franco OrtegaORCiD, Professor Neil Boonham
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Wiley-Blackwell, 2018.
For re-use rights please refer to the publisher's terms and conditions.
© 2018 British Society for Plant Pathology. Fusarium oxysporum f. sp. lactucae (FOL) is a soil- and seedborne pathogen and the causal agent of fusarium wilt on lettuce. Four races have been identified within FOL, with different worldwide distribution. Several molecular techniques have been used to detect and identify this pathogen; however, not all of them have the optimal characteristics in terms of sensitivity to perform FOL detection in plant and seed material. A loop-mediated isothermal amplification (LAMP) assay was developed based on the sequence-characterized amplified region (SCAR) obtained in a previous rapid amplification of polymorphic DNA (RAPD) study. The LAMP assay has been validated according to the EPPO standard PM7/98. The LAMP assay was tested with lettuce seeds, soil and plant material, and can be used successfully to amplify DNA from each of these matrices. In seed lots artificially inoculated with FOL, the detection limit of the LAMP test was 0.004% infected seed. Plant Pathology
Author(s): Franco Ortega S, Tomlinson J, Gilardi G, Spadaro D, Gullino ML, Garibaldi A, Boonham N
Publication type: Article
Publication status: Published
Journal: Plant Pathology
Year: 2018
Volume: 67
Issue: 7
Pages: 1462-1473
Print publication date: 01/09/2018
Online publication date: 14/03/2018
Acceptance date: 02/04/2016
Date deposited: 05/06/2018
ISSN (print): 0032-0862
ISSN (electronic): 1365-3059
Publisher: Wiley-Blackwell
URL: https://doi.org/10.1111/ppa.12855
DOI: 10.1111/ppa.12855
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