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Lookup NU author(s): Professor Brian Walker
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Current understanding of in vivo human brown adipose tissue (BAT) physiology is limited by a reliance on positron emission tomography (PET)/computed tomography (CT) scanning, which has measured exogenous glucose and fatty acid uptake but not quantified endogenous substrate utilization by BAT. Six lean, healthy men underwent 18fluorodeoxyglucose-PET/CT scanning to localize BAT so microdialysis catheters could be inserted in supraclavicular BAT under CT guidance and in abdominal subcutaneous white adipose tissue (WAT). Arterial and dialysate samples were collected during warm (~25°C) and cold exposure (~17°C), and blood flow was measured by 133xenon washout. During warm conditions, there was increased glucose uptake and lactate release and decreased glycerol release by BAT compared with WAT. Cold exposure increased blood flow, glycerol release, and glucose and glutamate uptake only by BAT. This novel use of microdialysis reveals that human BAT is metabolically active during warm conditions. BAT activation substantially increases local lipolysis but also utilization of other substrates such as glutamate.
Author(s): Weir G, Ramage LE, Akyol N, Rhodes JK, Kyle CJ, Fletcher AM, Craven TH, Wakelin SJ, Drake AJ, Gregoriades M-L, Ashton C, Weir N, van Beek EJR, Karpe F, Walker BR, Stimson RH
Publication type: Article
Publication status: Published
Journal: Cell Metabolism
Print publication date: 05/06/2018
Online publication date: 24/05/2018
Acceptance date: 27/04/2018
Date deposited: 04/05/2018
ISSN (print): 1550-4131
ISSN (electronic): 1932-7420
Publisher: Cell Press
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