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Inter-laboratory skin distribution study of 4-n-butyl resorcinol: The importance of liquid chromatography/mass spectrometry (HPLC–MS/MS) bioanalytical validation

Lookup NU author(s): Dr Simon Wilkinson

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Abstract

© 2017 Elsevier B.V. In the present study, three laboratories independently compared percutaneous absorption and distribution of 4-n-butylresorcinol, using human skin from five donors. Each laboratory used the same protocol for percutaneous absorption studies but different LC–MS/MS analytical methods to quantify the test compound. All laboratories respected the mass balance criteria (i.e. 100 ± 15%; average 96.5–102% of applied dose). Regarding usual inter-lab variability, good agreement was observed for all compartments with the greatest difference in the epidermis: 3.3 fold increase. The data obtained demonstrate that robustness of skin absorption data rely on properly validated analytical methods including sample extraction and LC–MS/MS method. It also includes clearly defined cutaneous absorption protocol for dose skin preparation, application, washing and tape stripping.


Publication metadata

Author(s): Wargniez W, Jungman E, Wilkinson S, Seyler N, Gregoire S

Publication type: Article

Publication status: Published

Journal: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Year: 2017

Volume: 1060

Pages: 416-423

Print publication date: 15/08/2017

Online publication date: 26/05/2017

Acceptance date: 25/05/2017

ISSN (print): 1570-0232

ISSN (electronic): 1873-376X

Publisher: Elsevier BV

URL: https://doi.org/10.1016/j.jchromb.2017.05.026

DOI: 10.1016/j.jchromb.2017.05.026

PubMed id: 28675855


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