Toggle Main Menu Toggle Search

Open Access padlockePrints

Crystal structure of the Acinetobacter baumannii outer membrane protein Omp33

Lookup NU author(s): Dr Javier Abellon-RuizORCiD, Dr Michael Zahn, Dr Arnaud Basle, Professor Bert van den Berg

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

© 2018 International Union of Crystallography. Acinetobacter baumannii is becoming a major threat to human health due to its multidrug resistance. This is owing in a large part to the low permeability of its outer membrane (OM), which prevents high internal antibiotic concentrations and makes antibiotic-resistance mechanisms more effective. To exploit OM channels as potential delivery vehicles for future antibiotics, structural information is required. One abundant OM protein in A. baumannii is Omp33. This protein has been reported to be important for the in vivo fitness and virulence of A. baumannii, but its structure is not known. Here, the X-ray crystal structure of Omp33 is reported at a resolution of 2.1 Å. Omp33 has a 14-β-stranded barrel without stable extracellular loop constrictions. Instead, an extended and unusual periplasmic turn connecting β-strands 2 and 3 is present, which folds into the pore lumen and completely blocks the aqueous channel. The Omp33 structure helps in understanding how A. baumannii OM proteins contribute to the low permeability of the cell envelope of this bacterium and suggests that Omp33 might function as a gated channel.


Publication metadata

Author(s): Abellon-Ruiz J, Zahn M, Basle A, van den Berg B

Publication type: Article

Publication status: Published

Journal: Acta Crystallographica Section D: Structural Biology

Year: 2018

Volume: 74

Issue: 9

Pages: 852-860

Online publication date: 01/09/2018

Acceptance date: 20/06/2018

ISSN (electronic): 2059-7983

Publisher: Wiley-Blackwell

URL: https://doi.org/10.1107/S205979831800904X

DOI: 10.1107/S205979831800904X


Altmetrics

Altmetrics provided by Altmetric


Share