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Lookup NU author(s): Dr Reia Guppy,
Professor John BythellORCiD
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Springer Verlag, 2019.
For re-use rights please refer to the publisher's terms and conditions.
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. The surface mucus layer (SML) is of critical importance in health, disease, and stress responses of corals; however, visualising the intact SML is challenging. Dehydration during histological preparation causes shrinkage and deformation of the mucus gel layer, while fragile, unattached mucus exudates are typically lost altogether. Here, we describe a novel technique using water-soluble glycol methacrylate resin embedding that more accurately preserves the in situ SML. Thickness of the preserved SML is similar to that previously measured on live corals using a glass microprobe. The technique allows microscopic visualisation of the SML structure, as well as thickness and continuity measurements, which are important indicators of SML function in health and disease.
Author(s): Guppy R, Brown B, Bythell JC
Publication type: Article
Publication status: Published
Journal: Coral Reefs
Print publication date: 01/06/2019
Online publication date: 23/03/2019
Acceptance date: 18/03/2019
Date deposited: 04/06/2019
ISSN (print): 0722-4028
ISSN (electronic): 1432-0975
Publisher: Springer Verlag
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