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Lookup NU author(s): Professor Brian Walker
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
The presence of estrogens, androgens and glucocorticoids as well as their receptors and steroid converting enzymes in adipose tissue has been established. Their contribution to diseases such as obesity, diabetes and hormone-dependent cancers is an active area of research. Our objective was to develop a LC-MS/MS method to quantify bioactive estrogens and glucocorticoids simultaneously in human adipose tissue. Estrogens and glucocorticoids were extracted from adipose tissue samples using solid-phase extraction. Estrogens were derivatized using 1-(5-fluoro-2,4-dinitrophenyl)-4-methylpiperazine (PPZ) and methyl iodide to generate a permanently charged molecule (MPPZ). Steroids were separated and quantified by LC-MS/MS. The limit of quantitation for the steroids was between 15 and 100 pg per sample. Accuracy and precision were acceptable (< 20%). Using this method, estradiol, estrone, cortisone and cortisol were quantified in adipose tissue from women with and without breast cancer. This novel assay of estrogens and glucocorticoids by LC-MS/MS coupled with derivatization allowed simultaneous quantification of a panel of steroids in human adipose tissue across the endogenous range of concentrations encountered in health and disease.
Author(s): Laforest S, Pelletier M, Denver N, Poirier B, Nguyen S, Walker BR, Durocher F, Homer NZM, Diorio C, Tchernof A, Andrew R
Publication type: Article
Publication status: Published
Journal: Journal of Steroid Biochemistry and Molecular Biology
Print publication date: 01/12/2019
Online publication date: 24/09/2019
Acceptance date: 18/09/2019
Date deposited: 19/09/2019
ISSN (print): 0960-0760
ISSN (electronic): 1879-1220
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