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Tracking elusive cargo: Illuminating spatio-temporal Type 3 effector protein dynamics using reporters

Lookup NU author(s): James Connolly

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2017 The Authors Cellular Microbiology Published by John Wiley & Sons LtdType 3 secretion systems form an integral part of the arsenal of many pathogenic bacteria. These injection machines, together with their cargo of subversive effector proteins, are capable of manipulating the cellular environment of the host in order to ensure persistence of the pathogen. In order to fully appreciate the functions of Type 3 effectors, it is necessary to gain spatio-temporal knowledge of each effector during the process of infection. A number of genetic modifications have been exploited in order to reveal effector protein secretion, translocation and subsequent activity, and localisation within host cells. In this review, we will discuss the many available approaches for tracking effector protein dynamics and discuss the challenges faced to improve the current technologies and gain a clearer picture of effector protein function.


Publication metadata

Author(s): O'Boyle N, Connolly JPR, Roe AJ

Publication type: Review

Publication status: Published

Journal: Cellular Microbiology

Year: 2018

Volume: 20

Issue: 1

Print publication date: 01/01/2018

Online publication date: 31/10/2017

Acceptance date: 26/10/2017

ISSN (print): 1462-5814

ISSN (electronic): 1462-5822

Publisher: Blackwell Publishing Ltd

URL: https://doi.org/10.1111/cmi.12797

DOI: 10.1111/cmi.12797

PubMed id: 29087624


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